Hi,
Just having an issue with making up a solution and don't trust myself that i am doing it properly!

My protocol says that i need to prepare 0.05M Tris HCL buffer PH 7.4, containing 1.0M NACL, 20mM EDTA, 1mM PMSF.

I need to make 3ml in total.

I have 1M stock solution of Tris HCL buffer PH 7.4, and EDTA, NACL, and PMSF in solid form.

I am thinking that I probably do not need the NACL as its purpose was probably to adjust the PH to the desired level but as mine is already at 7.4 i might not need it.

Does anybody know the right quantities to make up this solution or can offer any advice in this area i would greatly appreciate it.

Thanks

Siobhan